Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2969209..2969811 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N4S17_RS14425 Protein ID WP_000897305.1
Coordinates 2969209..2969520 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N4S17_RS14430 Protein ID WP_000356397.1
Coordinates 2969521..2969811 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS14400 (2965123) 2965123..2965722 + 600 WP_001295269.1 glucose-1-phosphatase -
N4S17_RS14405 (2965716) 2965716..2966588 + 873 WP_000920762.1 virulence factor BrkB family protein -
N4S17_RS14410 (2966585) 2966585..2967022 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N4S17_RS14415 (2967067) 2967067..2968008 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N4S17_RS14420 (2968072) 2968072..2968980 - 909 WP_001162704.1 alpha/beta hydrolase -
N4S17_RS14425 (2969209) 2969209..2969520 + 312 WP_000897305.1 hypothetical protein Toxin
N4S17_RS14430 (2969521) 2969521..2969811 + 291 WP_000356397.1 NadS family protein Antitoxin
N4S17_RS14435 (2970397) 2970397..2970615 + 219 WP_001295676.1 CopG family transcriptional regulator -
N4S17_RS14440 (2970834) 2970834..2971076 + 243 WP_001086388.1 protein YiiF -
N4S17_RS14445 (2971305) 2971305..2972285 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
N4S17_RS14450 (2972684) 2972684..2973613 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
N4S17_RS14455 (2973610) 2973610..2974245 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2971305..2972285 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T258002 WP_000897305.1 NZ_CP104472:2969209-2969520 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT258002 WP_000356397.1 NZ_CP104472:2969521-2969811 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References