Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2161919..2162718 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag N4S17_RS10505 Protein ID WP_000347266.1
Coordinates 2162254..2162718 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N4S17_RS10500 Protein ID WP_001307405.1
Coordinates 2161919..2162254 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS10485 (2157704) 2157704..2158474 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
N4S17_RS10490 (2158490) 2158490..2159824 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N4S17_RS10495 (2160199) 2160199..2161770 + 1572 WP_001273741.1 galactarate dehydratase -
N4S17_RS10500 (2161919) 2161919..2162254 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N4S17_RS10505 (2162254) 2162254..2162718 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N4S17_RS10510 (2162773) 2162773..2163582 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N4S17_RS10515 (2163831) 2163831..2165111 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
N4S17_RS10520 (2165134) 2165134..2165607 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N4S17_RS10525 (2165618) 2165618..2166397 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N4S17_RS10530 (2166387) 2166387..2167265 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N4S17_RS10535 (2167283) 2167283..2167717 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2151263..2162718 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T258000 WP_000347266.1 NZ_CP104472:2162254-2162718 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT258000 WP_001307405.1 NZ_CP104472:2161919-2162254 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References