Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 853318..853820 Replicon chromosome
Accession NZ_CP104472
Organism Escherichia coli strain UC4224

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag N4S17_RS04335 Protein ID WP_000767828.1
Coordinates 853318..853572 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag N4S17_RS04340 Protein ID WP_001259255.1
Coordinates 853569..853820 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4S17_RS04305 (848333) 848333..848560 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
N4S17_RS04310 (848574) 848574..849632 - 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
N4S17_RS04315 (849811) 849811..851169 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
N4S17_RS04320 (851159) 851159..851221 - 63 WP_010723108.1 membrane protein YoeI -
N4S17_RS04325 (851436) 851436..852365 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
N4S17_RS04330 (852411) 852411..853235 - 825 WP_000754758.1 SDR family oxidoreductase -
N4S17_RS04335 (853318) 853318..853572 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
N4S17_RS04340 (853569) 853569..853820 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
N4S17_RS04345 (854103) 854103..854153 + 51 WP_001364200.1 his operon leader peptide -
N4S17_RS04350 (854299) 854299..855198 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
N4S17_RS04355 (855204) 855204..856508 + 1305 WP_001493159.1 histidinol dehydrogenase -
N4S17_RS04360 (856505) 856505..857575 + 1071 WP_001564244.1 histidinol-phosphate transaminase -
N4S17_RS04365 (857575) 857575..858642 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T257994 WP_000767828.1 NZ_CP104472:c853572-853318 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT257994 WP_001259255.1 NZ_CP104472:c853820-853569 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References