257926

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 932193..932734 Replicon chromosome
Accession NZ_CP104442
Organism Escherichia coli strain HBUT-L

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag N1H43_RS04330 Protein ID WP_000615976.1
Coordinates 932193..932471 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N1H43_RS04335 Protein ID WP_000729704.1
Coordinates 932474..932734 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H43_RS04315 (929775) 929775..930353 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N1H43_RS04320 (930559) 930559..931326 + 768 WP_000333380.1 class II glutamine amidotransferase -
N1H43_RS04325 (931297) 931297..932037 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N1H43_RS04330 (932193) 932193..932471 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N1H43_RS04335 (932474) 932474..932734 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N1H43_RS04340 (932920) 932920..933693 + 774 WP_000543895.1 C40 family peptidase -
N1H43_RS04345 (933750) 933750..934106 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
N1H43_RS04350 (934099) 934099..934377 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
N1H43_RS04355 (934482) 934482..936575 - 2094 WP_001140808.1 flagellar biosynthesis protein FlhA -
N1H43_RS04360 (936559) 936559..937698 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 923952..934377 10425


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T257926 WP_000615976.1 NZ_CP104442:c932471-932193 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT257926 WP_000729704.1 NZ_CP104442:c932734-932474 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References