Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3636967..3637642 Replicon chromosome
Accession NZ_CP104405
Organism Enterobacter hormaechei subsp. steigerwaltii strain 4236

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag N5F16_RS17470 Protein ID WP_015571638.1
Coordinates 3636967..3637266 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag N5F16_RS17475 Protein ID WP_015571639.1
Coordinates 3637277..3637642 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F16_RS17460 (N5F16_17460) 3633344..3635536 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
N5F16_RS17465 (N5F16_17465) 3635517..3636716 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
N5F16_RS17470 (N5F16_17470) 3636967..3637266 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5F16_RS17475 (N5F16_17475) 3637277..3637642 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
N5F16_RS17480 (N5F16_17480) 3637669..3638850 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
N5F16_RS17485 (N5F16_17485) 3638871..3639758 - 888 WP_015571641.1 LysR family transcriptional regulator -
N5F16_RS17490 (N5F16_17490) 3639856..3640458 + 603 WP_017382759.1 short chain dehydrogenase -
N5F16_RS17495 (N5F16_17495) 3640455..3641186 - 732 WP_039269965.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T257914 WP_015571638.1 NZ_CP104405:3636967-3637266 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT257914 WP_015571639.1 NZ_CP104405:3637277-3637642 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References