Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1240313..1241040 Replicon chromosome
Accession NZ_CP104405
Organism Enterobacter hormaechei subsp. steigerwaltii strain 4236

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag N5F16_RS05925 Protein ID WP_033486526.1
Coordinates 1240729..1241040 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag N5F16_RS05920 Protein ID WP_045349893.1
Coordinates 1240313..1240732 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F16_RS05910 (N5F16_05910) 1238143..1239264 - 1122 WP_015571201.1 YbdK family carboxylate-amine ligase -
N5F16_RS05915 (N5F16_05915) 1239370..1240260 + 891 WP_047642348.1 oxidoreductase -
N5F16_RS05920 (N5F16_05920) 1240313..1240732 - 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
N5F16_RS05925 (N5F16_05925) 1240729..1241040 - 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
N5F16_RS05930 (N5F16_05930) 1241394..1241663 - 270 WP_047717959.1 hypothetical protein -
N5F16_RS05935 (N5F16_05935) 1241859..1242461 + 603 WP_023320086.1 site-specific integrase -
N5F16_RS05940 (N5F16_05940) 1242598..1243266 + 669 WP_023296073.1 epoxyqueuosine reductase QueH -
N5F16_RS05945 (N5F16_05945) 1243490..1243684 - 195 WP_226883114.1 hypothetical protein -
N5F16_RS05950 (N5F16_05950) 1243975..1244856 - 882 WP_003858850.1 LysR family transcriptional regulator -
N5F16_RS05955 (N5F16_05955) 1244962..1245741 + 780 WP_026080550.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T257905 WP_033486526.1 NZ_CP104405:c1241040-1240729 [Enterobacter hormaechei subsp. steigerwaltii]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT257905 WP_045349893.1 NZ_CP104405:c1240732-1240313 [Enterobacter hormaechei subsp. steigerwaltii]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References