Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1211577..1212304 Replicon chromosome
Accession NZ_CP104403
Organism Enterobacter hormaechei subsp. steigerwaltii strain csa13R

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag N5F14_RS05790 Protein ID WP_033486526.1
Coordinates 1211993..1212304 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag N5F14_RS05785 Protein ID WP_045349893.1
Coordinates 1211577..1211996 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F14_RS05775 (N5F14_05775) 1209407..1210528 - 1122 WP_015571201.1 YbdK family carboxylate-amine ligase -
N5F14_RS05780 (N5F14_05780) 1210634..1211524 + 891 WP_047642348.1 oxidoreductase -
N5F14_RS05785 (N5F14_05785) 1211577..1211996 - 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
N5F14_RS05790 (N5F14_05790) 1211993..1212304 - 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
N5F14_RS05795 (N5F14_05795) 1212658..1212927 - 270 WP_047717959.1 hypothetical protein -
N5F14_RS05800 (N5F14_05800) 1213123..1213725 + 603 WP_023320086.1 site-specific integrase -
N5F14_RS05805 (N5F14_05805) 1213862..1214530 + 669 WP_023296073.1 epoxyqueuosine reductase QueH -
N5F14_RS05810 (N5F14_05810) 1214754..1214948 - 195 WP_226883114.1 hypothetical protein -
N5F14_RS05815 (N5F14_05815) 1215239..1216120 - 882 WP_003858850.1 LysR family transcriptional regulator -
N5F14_RS05820 (N5F14_05820) 1216226..1217005 + 780 WP_026080550.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T257886 WP_033486526.1 NZ_CP104403:c1212304-1211993 [Enterobacter hormaechei subsp. steigerwaltii]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT257886 WP_045349893.1 NZ_CP104403:c1211996-1211577 [Enterobacter hormaechei subsp. steigerwaltii]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References