Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3664500..3665175 Replicon chromosome
Accession NZ_CP104401
Organism Enterobacter hormaechei subsp. steigerwaltii strain colR

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag N5F15_RS17655 Protein ID WP_015571638.1
Coordinates 3664500..3664799 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag N5F15_RS17660 Protein ID WP_015571639.1
Coordinates 3664810..3665175 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N5F15_RS17645 (N5F15_17645) 3660877..3663069 + 2193 WP_017692801.1 type I secretion system permease/ATPase -
N5F15_RS17650 (N5F15_17650) 3663050..3664249 + 1200 WP_017382757.1 HlyD family efflux transporter periplasmic adaptor subunit -
N5F15_RS17655 (N5F15_17655) 3664500..3664799 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N5F15_RS17660 (N5F15_17660) 3664810..3665175 + 366 WP_015571639.1 HigA family addiction module antitoxin Antitoxin
N5F15_RS17665 (N5F15_17665) 3665202..3666383 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
N5F15_RS17670 (N5F15_17670) 3666404..3667291 - 888 WP_015571641.1 LysR family transcriptional regulator -
N5F15_RS17675 (N5F15_17675) 3667389..3667991 + 603 WP_017382759.1 short chain dehydrogenase -
N5F15_RS17680 (N5F15_17680) 3667988..3668719 - 732 WP_039269965.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3660877..3686130 25253


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T257877 WP_015571638.1 NZ_CP104401:3664500-3664799 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT257877 WP_015571639.1 NZ_CP104401:3664810-3665175 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References