Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3804172..3804713 Replicon chromosome
Accession NZ_CP104384
Organism Escherichia coli strain JNQH498

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KIG00_RS18760 Protein ID WP_000615983.1
Coordinates 3804435..3804713 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KIG00_RS18755 Protein ID WP_000729703.1
Coordinates 3804172..3804432 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIG00_RS18740 (3800223) 3800223..3802316 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
KIG00_RS18745 (3802540) 3802540..3803037 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KIG00_RS18750 (3803213) 3803213..3803962 - 750 WP_000056849.1 C40 family peptidase -
KIG00_RS18755 (3804172) 3804172..3804432 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KIG00_RS18760 (3804435) 3804435..3804713 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KIG00_RS18765 (3804869) 3804869..3805609 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KIG00_RS18770 (3805580) 3805580..3806347 - 768 WP_000333380.1 class II glutamine amidotransferase -
KIG00_RS18775 (3806553) 3806553..3807131 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / clpV/tssH / tssA / tssA / hcp1/tssD1 3803213..3829959 26746


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T257848 WP_000615983.1 NZ_CP104384:3804435-3804713 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT257848 WP_000729703.1 NZ_CP104384:3804172-3804432 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References