Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3977942..3978539 Replicon chromosome
Accession NZ_CP104373
Organism Klebsiella pneumoniae strain 2021CK-01815

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag N4558_RS19170 Protein ID WP_004142563.1
Coordinates 3978222..3978539 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag N4558_RS19165 Protein ID WP_004142561.1
Coordinates 3977942..3978229 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4558_RS19135 (N4558_19130) 3974022..3974270 + 249 WP_002893037.1 DUF1158 domain-containing protein -
N4558_RS19140 (N4558_19135) 3974288..3974629 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
N4558_RS19145 (N4558_19140) 3974660..3975775 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
N4558_RS19150 (N4558_19145) 3975955..3976536 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
N4558_RS19155 (N4558_19150) 3976536..3976904 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
N4558_RS19160 (N4558_19155) 3977024..3977677 + 654 WP_019704893.1 oxygen-insensitive NAD(P)H nitroreductase -
N4558_RS19165 (N4558_19160) 3977942..3978229 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
N4558_RS19170 (N4558_19165) 3978222..3978539 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N4558_RS19175 (N4558_19170) 3978724..3979767 - 1044 WP_019704892.1 DUF2157 domain-containing protein -
N4558_RS19180 (N4558_19175) 3980437..3981303 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
N4558_RS19185 (N4558_19180) 3981412..3982839 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T257823 WP_004142563.1 NZ_CP104373:c3978539-3978222 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT257823 WP_004142561.1 NZ_CP104373:c3978229-3977942 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References