Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4057700..4058394 Replicon chromosome
Accession NZ_CP104371
Organism Escherichia coli strain 2021CK-01784

Toxin (Protein)


Gene name yafO Uniprot ID V0YKG6
Locus tag N4502_RS19505 Protein ID WP_001263485.1
Coordinates 4057996..4058394 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N4502_RS19500 Protein ID WP_000554758.1
Coordinates 4057700..4057993 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4502_RS19480 (4053117) 4053117..4053614 + 498 WP_000006263.1 REP-associated tyrosine transposase RayT -
N4502_RS19485 (4054053) 4054053..4055765 - 1713 Protein_3800 flagellar biosynthesis protein FlhA -
N4502_RS19490 (4055737) 4055737..4056522 + 786 WP_000207554.1 putative lateral flagellar export/assembly protein LafU -
N4502_RS19495 (4056593) 4056593..4057648 + 1056 WP_001226177.1 DNA polymerase IV -
N4502_RS19500 (4057700) 4057700..4057993 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N4502_RS19505 (4057996) 4057996..4058394 + 399 WP_001263485.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N4502_RS19510 (4058404) 4058404..4058856 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
N4502_RS19515 (4059102) 4059102..4059308 + 207 Protein_3806 RtcB family protein -
N4502_RS19520 (4059304) 4059304..4059656 + 353 Protein_3807 peptide chain release factor H -
N4502_RS19525 (4059713) 4059713..4061170 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
N4502_RS19530 (4061431) 4061431..4061889 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4062485) 4062485..4062565 + 81 NuclAT_11 - -
- (4062485) 4062485..4062565 + 81 NuclAT_11 - -
- (4062485) 4062485..4062565 + 81 NuclAT_11 - -
- (4062485) 4062485..4062565 + 81 NuclAT_11 - -
N4502_RS19535 (4061981) 4061981..4063225 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15418.84 Da        Isoelectric Point: 7.3840

>T257812 WP_001263485.1 NZ_CP104371:4057996-4058394 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257812 WP_000554758.1 NZ_CP104371:4057700-4057993 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YKG6

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References