Detailed information of TA system TA025781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1244746..1245323
• Replicon: chromosome
• Accession: NC_016631
• Organism: Granulicella mallensis MP5ACTX8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ACIX8_RS05230
• Protein ID: WP_044177849.1
• Coordinates: 1245042..1245323 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACIX8_RS05225
• Protein ID: WP_014264279.1
• Coordinates: 1244746..1245030 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIX8_RS05200	1240069..1240269	+	201	WP_013581125.1	cold-shock protein	-
ACIX8_RS05205	1240472..1241224	+	753	WP_014264275.1	3-oxoacyl-ACP reductase FabG	-
ACIX8_RS05210	1241337..1241942	+	606	WP_014264276.1	TetR/AcrR family transcriptional regulator	-
ACIX8_RS05215	1241950..1242951	-	1002	WP_014264277.1	hypothetical protein	-
ACIX8_RS05220	1243061..1244716	+	1656	WP_150110775.1	MFS transporter	-
ACIX8_RS05225	1244746..1245030	-	285	WP_014264279.1	HigA family addiction module antidote protein	Antitoxin
ACIX8_RS05230	1245042..1245323	-	282	WP_044177849.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIX8_RS05235	1245403..1247922	-	2520	WP_014264281.1	2-oxoglutarate dehydrogenase E1 component	-
ACIX8_RS05240	1248410..1249135	+	726	WP_150110498.1	prolipoprotein diacylglyceryl transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1238766..1260425	21659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10655.15 Da
• Isoelectric Point: 8.1062

>T25781 WP_044177849.1 NC_016631:c1245323-1245042 [Granulicella mallensis MP5ACTX8]
VIVSFRDADTALLWDTGKSRRIPANIHISAWKKLAILNAALELENLTVPPGNRLEALKGDRKGQHSIRVNDQYRVCFIWH
DKNAHDVEIVDYH

Nucleotide

• Length: 282 bp

>T25781 NC_016631:c1245323-1245042 [Granulicella mallensis MP5ACTX8]
GTGATTGTGAGCTTCCGAGATGCCGATACGGCGTTGCTGTGGGACACAGGCAAAAGCCGCCGTATTCCTGCAAACATCCA
CATATCGGCATGGAAGAAACTCGCAATTCTCAATGCCGCTCTCGAACTGGAAAACCTGACAGTTCCACCAGGCAACAGGC
TTGAGGCATTGAAAGGTGATCGTAAAGGTCAACATAGTATCCGGGTGAACGATCAATATCGTGTGTGTTTCATCTGGCAC
GATAAGAACGCGCACGATGTAGAGATCGTGGACTACCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10518.30 Da
• Isoelectric Point: 10.0367

>AT25781 WP_014264279.1 NC_016631:c1245030-1244746 [Granulicella mallensis MP5ACTX8]
MPQPFAIHPGDILLTEFMEPLGLTAYRLAKDLHVSVPRMNDVVRGKRSISADTALRLGIYFGLPAQFWLNLQNDYDLRLA
KSNALAKVQPRLAA

Nucleotide

• Length: 285 bp

>AT25781 NC_016631:c1245030-1244746 [Granulicella mallensis MP5ACTX8]
ATGCCCCAGCCATTCGCAATCCATCCTGGAGACATCCTGCTGACCGAGTTCATGGAACCACTCGGTCTCACGGCATACCG
TCTCGCAAAGGACCTGCATGTCTCTGTTCCCCGCATGAACGACGTAGTGCGAGGCAAGCGTTCCATTTCAGCCGATACTG
CACTTCGGCTCGGCATCTACTTCGGACTACCGGCACAGTTCTGGCTGAATCTCCAGAACGACTATGACCTCCGCCTTGCT
AAGAGTAACGCGTTAGCAAAAGTACAACCGCGGCTTGCGGCCTAG