Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2299363..2300162 Replicon chromosome
Accession NZ_CP104371
Organism Escherichia coli strain 2021CK-01784

Toxin (Protein)


Gene name yhaV Uniprot ID A0A369FDQ4
Locus tag N4502_RS11155 Protein ID WP_000347280.1
Coordinates 2299698..2300162 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N4502_RS11150 Protein ID WP_001307405.1
Coordinates 2299363..2299698 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4502_RS11135 (2295148) 2295148..2295918 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
N4502_RS11140 (2295934) 2295934..2297268 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
N4502_RS11145 (2297643) 2297643..2299214 + 1572 WP_001273738.1 galactarate dehydratase -
N4502_RS11150 (2299363) 2299363..2299698 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N4502_RS11155 (2299698) 2299698..2300162 + 465 WP_000347280.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N4502_RS11160 (2300217) 2300217..2301026 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
N4502_RS11165 (2301275) 2301275..2302555 + 1281 WP_000681960.1 tagatose-bisphosphate aldolase subunit KbaZ -
N4502_RS11170 (2302578) 2302578..2303051 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N4502_RS11175 (2303062) 2303062..2303841 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N4502_RS11180 (2303831) 2303831..2304709 + 879 WP_039720218.1 PTS N-acetylgalactosamine transporter subunit IID -
N4502_RS11185 (2304727) 2304727..2305161 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2290123..2300162 10039


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17862.29 Da        Isoelectric Point: 9.6924

>T257806 WP_000347280.1 NZ_CP104371:2299698-2300162 [Escherichia coli]
MDFPQRVNSWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257806 WP_001307405.1 NZ_CP104371:2299363-2299698 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A369FDQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References