Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2254956..2255683 Replicon chromosome
Accession NZ_CP104371
Organism Escherichia coli strain 2021CK-01784

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N4502_RS10925 Protein ID WP_000550189.1
Coordinates 2255369..2255683 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag N4502_RS10920 Protein ID WP_000560253.1
Coordinates 2254956..2255372 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4502_RS10910 (2250371) 2250371..2252722 + 2352 WP_000695464.1 alpha-glucosidase -
N4502_RS10915 (2252893) 2252893..2254911 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N4502_RS10920 (2254956) 2254956..2255372 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N4502_RS10925 (2255369) 2255369..2255683 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N4502_RS10930 (2255968) 2255968..2257104 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N4502_RS10935 (2257189) 2257189..2257692 + 504 WP_001305999.1 M48 family metallopeptidase -
N4502_RS10940 (2257769) 2257769..2258452 + 684 WP_001183059.1 vancomycin high temperature exclusion protein -
N4502_RS10945 (2258531) 2258531..2259517 + 987 WP_001315023.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T257805 WP_000550189.1 NZ_CP104371:c2255683-2255369 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT257805 WP_000560253.1 NZ_CP104371:c2255372-2254956 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References