Detailed information of TA system TA025771

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 276963..277545
• Replicon: plasmid AZO_p3
• Accession: NC_016623
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AZOLI_RS31585
• Protein ID: WP_014249557.1
• Coordinates: 277267..277545 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AZOLI_RS22840
• Protein ID: WP_014249556.1
• Coordinates: 276963..277250 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS22820	272951..273487	+	537	WP_014249553.1	tripartite tricarboxylate transporter TctB family protein	-
AZOLI_RS22825	273539..275065	+	1527	WP_014249554.1	tripartite tricarboxylate transporter permease	-
AZOLI_RS22830	275102..275878	+	777	WP_014249555.1	SDR family oxidoreductase	-
AZOLI_RS22835	275958..276930	+	973	Protein_246	MBL fold metallo-hydrolase	-
AZOLI_RS22840	276963..277250	-	288	WP_014249556.1	HigA family addiction module antidote protein	Antitoxin
AZOLI_RS31585	277267..277545	-	279	WP_014249557.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZOLI_RS22850	277665..280256	-	2592	WP_014249558.1	bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase	-
AZOLI_RS22855	280459..280965	-	507	WP_014249559.1	ribonuclease E activity regulator RraA	-
AZOLI_RS22860	281169..281543	+	375	WP_014249560.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	adeG	1..648491	648491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10640.04 Da
• Isoelectric Point: 8.0650

>T25771 WP_014249557.1 NC_016623:c277545-277267 [Azospirillum lipoferum 4B]
MIRSFRDRQTKALYEGETVRQFTTVSKVAARKLDMLNAAAVLEDLRSPPGNRLEALKGDRAGQHSIRINDQWRLCFVWRE
DGAHDVEIVDYH

Nucleotide

• Length: 279 bp

>T25771 NC_016623:c277545-277267 [Azospirillum lipoferum 4B]
ATGATACGCAGCTTCCGTGACCGGCAGACCAAGGCGCTGTATGAGGGCGAGACCGTCCGGCAATTCACTACCGTCTCCAA
GGTCGCTGCGCGGAAACTGGACATGCTGAACGCCGCAGCGGTGTTGGAGGATCTGCGTTCGCCCCCGGGGAATCGACTGG
AAGCGCTCAAAGGAGATCGAGCCGGGCAGCACAGTATACGGATCAACGACCAATGGCGTCTCTGCTTCGTCTGGCGCGAG
GATGGCGCCCACGATGTAGAGATCGTCGACTACCATTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10501.94 Da
• Isoelectric Point: 6.8802

>AT25771 WP_014249556.1 NC_016623:c277250-276963 [Azospirillum lipoferum 4B]
MRIKTHPGEVLREEFMEPLGLTANALSIALRVPATRIGEIVNERRGISADTALRLARYFGTTPQFWLNLQSDHDLSVAAS
AHGAEIERDVHPRAA

Nucleotide

• Length: 288 bp

>AT25771 NC_016623:c277250-276963 [Azospirillum lipoferum 4B]
ATGCGCATCAAGACACATCCCGGCGAAGTGCTGCGGGAGGAGTTCATGGAGCCGCTGGGGCTGACGGCCAATGCGCTGTC
CATCGCCTTGCGGGTTCCCGCCACCCGGATCGGCGAGATCGTCAATGAACGTCGGGGCATCAGTGCCGATACCGCATTGC
GCCTCGCCCGTTACTTCGGCACCACGCCACAGTTCTGGCTGAACCTGCAATCGGACCATGACCTGTCGGTCGCCGCCAGT
GCGCATGGCGCGGAGATCGAACGCGACGTCCATCCCCGCGCAGCTTGA