Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 156964..157477 Replicon plasmid AZO_p3
Accession NC_016623
Organism Azospirillum lipoferum 4B

Toxin (Protein)


Gene name yoeB Uniprot ID G7ZF26
Locus tag AZOLI_RS22305 Protein ID WP_014249446.1
Coordinates 156964..157230 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A6N1ADX4
Locus tag AZOLI_RS22310 Protein ID WP_044552781.1
Coordinates 157211..157477 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZOLI_RS22280 152921..153763 + 843 WP_162488376.1 GntR family transcriptional regulator -
AZOLI_RS22285 153842..154660 + 819 WP_014249442.1 transporter substrate-binding domain-containing protein -
AZOLI_RS22290 154726..155373 + 648 WP_014249443.1 amino acid ABC transporter permease -
AZOLI_RS22295 155378..156082 + 705 WP_014249444.1 amino acid ABC transporter permease -
AZOLI_RS22300 156145..156909 + 765 WP_162488402.1 amino acid ABC transporter ATP-binding protein -
AZOLI_RS22305 156964..157230 - 267 WP_014249446.1 Txe/YoeB family addiction module toxin Toxin
AZOLI_RS22310 157211..157477 - 267 WP_044552781.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
AZOLI_RS22315 157651..158325 + 675 WP_014249448.1 ATP-binding cassette domain-containing protein -
AZOLI_RS22320 158325..158972 + 648 WP_014249449.1 energy-coupling factor transporter transmembrane protein EcfT -
AZOLI_RS22325 159003..159548 + 546 WP_044553011.1 biotin transporter BioY -
AZOLI_RS22330 159553..161040 + 1488 WP_044552784.1 AMP-binding protein -
AZOLI_RS22335 161037..162206 + 1170 WP_014249452.1 thiolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - adeG 1..648491 648491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10252.67 Da        Isoelectric Point: 9.4087

>T25770 WP_014249446.1 NC_016623:c157230-156964 [Azospirillum lipoferum 4B]
MKKVWTDGAWEDYLYWQKNDQAVLKKVNDLIRDTERNPFSGLGKPEPLKEGLAGWWSRRITGEHRLVYRVTGSGAAQALE
ILACRYHY

Download         Length: 267 bp

>T25770 NC_016623:c157230-156964 [Azospirillum lipoferum 4B]
ATGAAGAAGGTTTGGACTGACGGCGCTTGGGAGGATTACTTATACTGGCAGAAGAACGACCAGGCCGTGTTGAAGAAGGT
CAACGATCTGATCCGCGACACTGAAAGGAACCCGTTCTCAGGACTGGGCAAGCCTGAACCCCTGAAGGAAGGGCTTGCCG
GCTGGTGGTCGCGCCGCATCACGGGCGAACATCGGCTGGTCTATCGGGTGACCGGGAGCGGTGCCGCCCAAGCACTCGAA
ATACTGGCCTGCCGGTATCACTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9798.17 Da        Isoelectric Point: 4.6768

>AT25770 WP_044552781.1 NC_016623:c157477-157211 [Azospirillum lipoferum 4B]
MGHISYTDLRNNLASVMDEVCDSRAPLTVTRQKGRSVVMISEEEFEGLMETLHLLKSPKNAERLLKGIAQLDAGKGIERD
ILNEEGLD

Download         Length: 267 bp

>AT25770 NC_016623:c157477-157211 [Azospirillum lipoferum 4B]
ATGGGTCACATCAGCTACACCGATCTTAGAAACAACCTTGCCAGCGTGATGGACGAGGTCTGTGACAGCCGCGCGCCGCT
GACCGTCACCCGTCAGAAGGGACGGTCGGTGGTGATGATTTCCGAGGAAGAATTCGAAGGGCTGATGGAGACGCTTCATC
TCCTGAAAAGCCCCAAAAACGCGGAACGGCTGCTCAAGGGCATCGCCCAACTTGATGCGGGCAAAGGCATCGAGCGGGAC
ATTCTGAATGAAGAAGGTTTGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G7ZF26

Antitoxin

Source ID Structure
AlphaFold DB A0A6N1ADX4

References