257665

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3589735..3590429 Replicon chromosome
Accession NZ_CP104330
Organism Escherichia coli strain THB42-F3

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N0544_RS17575 Protein ID WP_001263489.1
Coordinates 3589735..3590133 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N0544_RS17580 Protein ID WP_000554758.1
Coordinates 3590136..3590429 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3585323) 3585323..3585403 - 81 NuclAT_12 - -
- (3585323) 3585323..3585403 - 81 NuclAT_12 - -
- (3585323) 3585323..3585403 - 81 NuclAT_12 - -
- (3585323) 3585323..3585403 - 81 NuclAT_12 - -
N0544_RS17550 (3585999) 3585999..3586457 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N0544_RS17555 (3586718) 3586718..3588175 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N0544_RS17560 (3588232) 3588232..3588753 - 522 Protein_3431 peptide chain release factor H -
N0544_RS17565 (3588749) 3588749..3588955 - 207 Protein_3432 RtcB family protein -
N0544_RS17570 (3589273) 3589273..3589725 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N0544_RS17575 (3589735) 3589735..3590133 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N0544_RS17580 (3590136) 3590136..3590429 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N0544_RS17585 (3590481) 3590481..3591536 - 1056 WP_001226164.1 DNA polymerase IV -
N0544_RS17590 (3591607) 3591607..3592392 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0544_RS17595 (3592364) 3592364..3594076 + 1713 Protein_3438 flagellar biosynthesis protein FlhA -
N0544_RS17600 (3594300) 3594300..3594797 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T257665 WP_001263489.1 NZ_CP104330:c3590133-3589735 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257665 WP_000554758.1 NZ_CP104330:c3590429-3590136 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References