Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3595036..3595577 Replicon chromosome
Accession NZ_CP104328
Organism Escherichia coli strain THB42-F2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag N0499_RS17605 Protein ID WP_000615983.1
Coordinates 3595299..3595577 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag N0499_RS17600 Protein ID WP_000729703.1
Coordinates 3595036..3595296 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0499_RS17580 (3590711) 3590711..3591496 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0499_RS17585 (3591468) 3591468..3593180 + 1713 Protein_3436 flagellar biosynthesis protein FlhA -
N0499_RS17590 (3593404) 3593404..3593901 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N0499_RS17595 (3594077) 3594077..3594826 - 750 WP_000056849.1 C40 family peptidase -
N0499_RS17600 (3595036) 3595036..3595296 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N0499_RS17605 (3595299) 3595299..3595577 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N0499_RS17610 (3595733) 3595733..3596473 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N0499_RS17615 (3596444) 3596444..3597211 - 768 WP_000333380.1 class II glutamine amidotransferase -
N0499_RS17620 (3597417) 3597417..3597995 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T257636 WP_000615983.1 NZ_CP104328:3595299-3595577 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT257636 WP_000729703.1 NZ_CP104328:3595036-3595296 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References