Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3588839..3589533 Replicon chromosome
Accession NZ_CP104328
Organism Escherichia coli strain THB42-F2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N0499_RS17565 Protein ID WP_001263489.1
Coordinates 3588839..3589237 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N0499_RS17570 Protein ID WP_000554758.1
Coordinates 3589240..3589533 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3584427) 3584427..3584507 - 81 NuclAT_12 - -
- (3584427) 3584427..3584507 - 81 NuclAT_12 - -
- (3584427) 3584427..3584507 - 81 NuclAT_12 - -
- (3584427) 3584427..3584507 - 81 NuclAT_12 - -
N0499_RS17540 (3585103) 3585103..3585561 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N0499_RS17545 (3585822) 3585822..3587279 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N0499_RS17550 (3587336) 3587336..3587857 - 522 Protein_3429 peptide chain release factor H -
N0499_RS17555 (3587853) 3587853..3588059 - 207 Protein_3430 RtcB family protein -
N0499_RS17560 (3588377) 3588377..3588829 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N0499_RS17565 (3588839) 3588839..3589237 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N0499_RS17570 (3589240) 3589240..3589533 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N0499_RS17575 (3589585) 3589585..3590640 - 1056 WP_001226164.1 DNA polymerase IV -
N0499_RS17580 (3590711) 3590711..3591496 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0499_RS17585 (3591468) 3591468..3593180 + 1713 Protein_3436 flagellar biosynthesis protein FlhA -
N0499_RS17590 (3593404) 3593404..3593901 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T257635 WP_001263489.1 NZ_CP104328:c3589237-3588839 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257635 WP_000554758.1 NZ_CP104328:c3589533-3589240 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References