Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3638158..3638699 Replicon chromosome
Accession NZ_CP104326
Organism Escherichia coli strain THB42-F1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag N0470_RS17830 Protein ID WP_000615983.1
Coordinates 3638421..3638699 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag N0470_RS17825 Protein ID WP_000729703.1
Coordinates 3638158..3638418 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0470_RS17805 (3633833) 3633833..3634618 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0470_RS17810 (3634590) 3634590..3636302 + 1713 Protein_3481 flagellar biosynthesis protein FlhA -
N0470_RS17815 (3636526) 3636526..3637023 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
N0470_RS17820 (3637199) 3637199..3637948 - 750 WP_000056849.1 C40 family peptidase -
N0470_RS17825 (3638158) 3638158..3638418 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N0470_RS17830 (3638421) 3638421..3638699 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N0470_RS17835 (3638855) 3638855..3639595 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N0470_RS17840 (3639566) 3639566..3640333 - 768 WP_000333380.1 class II glutamine amidotransferase -
N0470_RS17845 (3640539) 3640539..3641117 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T257605 WP_000615983.1 NZ_CP104326:3638421-3638699 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT257605 WP_000729703.1 NZ_CP104326:3638158-3638418 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References