257604

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3631961..3632655 Replicon chromosome
Accession NZ_CP104326
Organism Escherichia coli strain THB42-F1

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag N0470_RS17790 Protein ID WP_001263489.1
Coordinates 3631961..3632359 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N0470_RS17795 Protein ID WP_000554758.1
Coordinates 3632362..3632655 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3627549) 3627549..3627629 - 81 NuclAT_12 - -
- (3627549) 3627549..3627629 - 81 NuclAT_12 - -
- (3627549) 3627549..3627629 - 81 NuclAT_12 - -
- (3627549) 3627549..3627629 - 81 NuclAT_12 - -
N0470_RS17765 (3628225) 3628225..3628683 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
N0470_RS17770 (3628944) 3628944..3630401 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
N0470_RS17775 (3630458) 3630458..3630979 - 522 Protein_3474 peptide chain release factor H -
N0470_RS17780 (3630975) 3630975..3631181 - 207 Protein_3475 RtcB family protein -
N0470_RS17785 (3631499) 3631499..3631951 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
N0470_RS17790 (3631961) 3631961..3632359 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N0470_RS17795 (3632362) 3632362..3632655 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N0470_RS17800 (3632707) 3632707..3633762 - 1056 WP_001226164.1 DNA polymerase IV -
N0470_RS17805 (3633833) 3633833..3634618 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
N0470_RS17810 (3634590) 3634590..3636302 + 1713 Protein_3481 flagellar biosynthesis protein FlhA -
N0470_RS17815 (3636526) 3636526..3637023 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T257604 WP_001263489.1 NZ_CP104326:c3632359-3631961 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257604 WP_000554758.1 NZ_CP104326:c3632655-3632362 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References