Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4362099..4362793 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag NUE46_RS22175 Protein ID WP_001263495.1
Coordinates 4362099..4362497 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NUE46_RS22180 Protein ID WP_000554757.1
Coordinates 4362500..4362793 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4357686) 4357686..4357766 - 81 NuclAT_12 - -
- (4357686) 4357686..4357766 - 81 NuclAT_12 - -
- (4357686) 4357686..4357766 - 81 NuclAT_12 - -
- (4357686) 4357686..4357766 - 81 NuclAT_12 - -
NUE46_RS22150 (4358362) 4358362..4358820 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NUE46_RS22155 (4359081) 4359081..4360538 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
NUE46_RS22160 (4360595) 4360595..4361116 - 522 Protein_4341 peptide chain release factor H -
NUE46_RS22165 (4361115) 4361115..4361318 - 204 Protein_4342 RtcB family protein -
NUE46_RS22170 (4361637) 4361637..4362089 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
NUE46_RS22175 (4362099) 4362099..4362497 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NUE46_RS22180 (4362500) 4362500..4362793 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NUE46_RS22185 (4362845) 4362845..4363900 - 1056 WP_001226188.1 DNA polymerase IV -
NUE46_RS22190 (4363971) 4363971..4364756 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
NUE46_RS22195 (4364728) 4364728..4366440 + 1713 Protein_4348 flagellar biosynthesis protein FlhA -
NUE46_RS22200 (4366585) 4366585..4367082 - 498 Protein_4349 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T257548 WP_001263495.1 NZ_CP104309:c4362497-4362099 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT257548 WP_000554757.1 NZ_CP104309:c4362793-4362500 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References