Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 652566..653365 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NUE46_RS03245 Protein ID WP_000347273.1
Coordinates 652566..653030 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag NUE46_RS03250 Protein ID WP_001302819.1
Coordinates 653030..653365 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS03215 (647567) 647567..648001 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NUE46_RS03220 (648019) 648019..648897 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NUE46_RS03225 (648887) 648887..649666 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
NUE46_RS03230 (649677) 649677..650150 - 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
NUE46_RS03235 (650173) 650173..651453 - 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
NUE46_RS03240 (651702) 651702..652511 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NUE46_RS03245 (652566) 652566..653030 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NUE46_RS03250 (653030) 653030..653365 - 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NUE46_RS03255 (653514) 653514..655085 - 1572 WP_001273776.1 galactarate dehydratase -
NUE46_RS03260 (655460) 655460..656794 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NUE46_RS03265 (656810) 656810..657580 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T257531 WP_000347273.1 NZ_CP104309:c653030-652566 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT257531 WP_001302819.1 NZ_CP104309:c653365-653030 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References