25751

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1331096..1331746 Replicon chromosome
Accession NC_016612
Organism Klebsiella oxytoca KCTC 1686

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag KOX_RS06315 Protein ID WP_014227314.1
Coordinates 1331405..1331746 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag KOX_RS06310 Protein ID WP_014227313.1
Coordinates 1331096..1331395 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOX_RS06290 1326981..1327700 - 720 WP_014227309.1 phosphonate utilization transcriptional regulator PhnR -
KOX_RS06295 1327841..1328944 + 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
KOX_RS06300 1328954..1329763 + 810 WP_014227311.1 phosphonoacetaldehyde hydrolase -
KOX_RS06305 1329796..1330977 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
KOX_RS06310 1331096..1331395 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
KOX_RS06315 1331405..1331746 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KOX_RS06320 1331997..1332449 - 453 WP_009653860.1 DUF1198 domain-containing protein -
KOX_RS06325 1332898..1334289 - 1392 WP_014227315.1 hexose-6-phosphate:phosphate antiporter -
KOX_RS06330 1334435..1335754 - 1320 WP_014227316.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T25751 WP_014227314.1 NC_016612:c1331746-1331405 [Klebsiella michiganensis KCTC 1686]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T25751 NC_016612:c1331746-1331405 [Klebsiella michiganensis KCTC 1686]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT25751 WP_014227313.1 NC_016612:c1331395-1331096 [Klebsiella michiganensis KCTC 1686]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT25751 NC_016612:c1331395-1331096 [Klebsiella michiganensis KCTC 1686]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References