Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1168361..1169088 Replicon chromosome
Accession NZ_CP104284
Organism Enterobacter hormaechei subsp. steigerwaltii strain JT.WLB1A

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q486
Locus tag N2K85_RS05480 Protein ID WP_033486526.1
Coordinates 1168777..1169088 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q1H0
Locus tag N2K85_RS05475 Protein ID WP_045349893.1
Coordinates 1168361..1168780 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2K85_RS05465 (N2K85_05465) 1166191..1167312 - 1122 WP_047734301.1 YbdK family carboxylate-amine ligase -
N2K85_RS05470 (N2K85_05470) 1167418..1168308 + 891 WP_260673290.1 oxidoreductase -
N2K85_RS05475 (N2K85_05475) 1168361..1168780 - 420 WP_045349893.1 helix-turn-helix domain-containing protein Antitoxin
N2K85_RS05480 (N2K85_05480) 1168777..1169088 - 312 WP_033486526.1 type II toxin-antitoxin system HigB family toxin Toxin
N2K85_RS05485 (N2K85_05485) 1169540..1170019 + 480 WP_047734296.1 hypothetical protein -
N2K85_RS05490 (N2K85_05490) 1170392..1171684 + 1293 WP_047734294.1 tetratricopeptide repeat protein -
N2K85_RS05495 (N2K85_05495) 1171919..1172737 - 819 WP_047734292.1 DUF2971 domain-containing protein -
N2K85_RS05500 (N2K85_05500) 1172951..1173553 + 603 WP_047734291.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12506.30 Da        Isoelectric Point: 9.9435

>T257461 WP_033486526.1 NZ_CP104284:c1169088-1168777 [Enterobacter hormaechei subsp. steigerwaltii]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLTDYYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15375.45 Da        Isoelectric Point: 4.7132

>AT257461 WP_045349893.1 NZ_CP104284:c1168780-1168361 [Enterobacter hormaechei subsp. steigerwaltii]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVDMLTAKIDKYENASPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q486

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q1H0

References