257446

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3771947..3772641 Replicon chromosome
Accession NZ_CP104274
Organism Escherichia coli strain Ec15103

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag N2O69_RS18540 Protein ID WP_001263500.1
Coordinates 3771947..3772345 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag N2O69_RS18545 Protein ID WP_000554758.1
Coordinates 3772348..3772641 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2O69_RS18515 (3767312) 3767312..3767770 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
N2O69_RS18520 (3768031) 3768031..3769488 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
N2O69_RS18525 (3769545) 3769545..3770159 - 615 WP_000602124.1 peptide chain release factor H -
N2O69_RS18530 (3770156) 3770156..3771295 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
N2O69_RS18535 (3771485) 3771485..3771937 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
N2O69_RS18540 (3771947) 3771947..3772345 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N2O69_RS18545 (3772348) 3772348..3772641 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N2O69_RS18550 (3772693) 3772693..3773738 - 1046 Protein_3633 DNA polymerase IV -
N2O69_RS18555 (3773809) 3773809..3774732 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
N2O69_RS18560 (3774735) 3774735..3775598 - 864 WP_001169518.1 flagellar motor stator protein MotA -
N2O69_RS18565 (3775611) 3775611..3776327 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
N2O69_RS18570 (3776347) 3776347..3776814 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T257446 WP_001263500.1 NZ_CP104274:c3772345-3771947 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT257446 WP_000554758.1 NZ_CP104274:c3772641-3772348 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References