Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2467716..2468278 Replicon chromosome
Accession NZ_CP104274
Organism Escherichia coli strain Ec15103

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag N2O69_RS11960 Protein ID WP_000605675.1
Coordinates 2467716..2467994 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag N2O69_RS11965 Protein ID WP_000781370.1
Coordinates 2467994..2468278 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2O69_RS11935 (2463302) 2463302..2463733 - 432 WP_000152305.1 peroxiredoxin OsmC -
N2O69_RS11940 (2464078) 2464078..2464293 + 216 WP_000495766.1 biofilm-dependent modulation protein -
N2O69_RS11945 (2464395) 2464395..2464532 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
N2O69_RS11950 (2464689) 2464689..2466386 + 1698 WP_000433460.1 malate dehydrogenase -
N2O69_RS11955 (2466520) 2466520..2467530 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
N2O69_RS11960 (2467716) 2467716..2467994 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N2O69_RS11965 (2467994) 2467994..2468278 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
N2O69_RS11970 (2468507) 2468507..2469160 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
N2O69_RS11975 (2469153) 2469153..2470037 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
N2O69_RS11980 (2470050) 2470050..2473097 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T257443 WP_000605675.1 NZ_CP104274:2467716-2467994 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT257443 WP_000781370.1 NZ_CP104274:2467994-2468278 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References