Detailed information of TA system TA025738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 229979..230627
• Replicon: plasmid AZO_p6
• Accession: NC_016588
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: AZOLI_RS30020
• Protein ID: WP_044553855.1
• Coordinates: 230304..230627 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AZOLI_RS30015
• Protein ID: WP_014190039.1
• Coordinates: 229979..230296 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS29990	226027..226695	+	669	WP_014190034.1	DUF2076 domain-containing protein	-
AZOLI_RS29995	226785..228158	-	1374	WP_014190035.1	nucleotide sugar dehydrogenase	-
AZOLI_RS30000	228349..228711	+	363	WP_014190036.1	nucleotidyltransferase domain-containing protein	-
AZOLI_RS32825	228698..228856	+	159	WP_162488549.1	hypothetical protein	-
AZOLI_RS30005	228853..229101	+	249	WP_044553760.1	HEPN domain-containing protein	-
AZOLI_RS30010	229110..229982	+	873	WP_014190038.1	hypothetical protein	-
AZOLI_RS30015	229979..230296	-	318	WP_014190039.1	HigA family addiction module antidote protein	Antitoxin
AZOLI_RS30020	230304..230627	-	324	WP_044553855.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZOLI_RS30025	231108..231332	+	225	WP_014190041.1	hypothetical protein	-
AZOLI_RS30030	231457..231921	+	465	WP_014190042.1	translation initiation factor IF-3	-
AZOLI_RS30035	232217..233860	+	1644	WP_014190043.1	PAS domain-containing sensor histidine kinase	-
AZOLI_RS30040	233986..235212	+	1227	WP_014190044.1	D-alanyl-D-alanine carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..295744	295744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-80)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12188.91 Da
• Isoelectric Point: 9.5555

>T25738 WP_044553855.1 NC_016588:c230627-230304 [Azospirillum lipoferum 4B]
MPIRNCADRRTADFLAGKRVKEFQSFERQAAKALTKLQAATRLMDLRYPPSNRFEALGGERKGQYSIRINDQWRVCFRWA
VIGGAQADADLLTVPGEPYDVVIADYH

Nucleotide

• Length: 324 bp

>T25738 NC_016588:c230627-230304 [Azospirillum lipoferum 4B]
ATGCCGATAAGAAACTGCGCGGACAGACGGACTGCCGATTTCCTGGCCGGCAAGCGGGTGAAGGAGTTCCAGTCCTTCGA
GCGCCAAGCCGCCAAGGCATTGACCAAGCTCCAGGCGGCCACCCGGCTGATGGATCTCCGGTACCCTCCGTCCAATCGCT
TCGAAGCGCTGGGCGGGGAACGGAAGGGCCAGTACAGCATCCGCATCAACGACCAGTGGCGAGTCTGTTTCCGCTGGGCG
GTGATTGGGGGCGCGCAGGCAGACGCCGACCTCCTGACCGTTCCAGGAGAGCCCTACGACGTCGTGATCGCCGATTACCA
CTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11436.06 Da
• Isoelectric Point: 6.4823

>AT25738 WP_014190039.1 NC_016588:c230296-229979 [Azospirillum lipoferum 4B]
MSHPATDNLPPIHPGEFLRDELEALGLSARRFAEHIGVPPNAVTEIMNGERRITAPMALRLGKAFGTDARYWTNLQSIFE
TKVARAEIGDALDAIQPLPHPGKAA

Nucleotide

• Length: 318 bp

>AT25738 NC_016588:c230296-229979 [Azospirillum lipoferum 4B]
ATGAGCCATCCCGCCACCGACAATCTGCCGCCCATTCATCCCGGCGAGTTCCTGCGTGACGAGTTGGAGGCGCTGGGCCT
GAGCGCCCGGCGGTTCGCCGAGCATATCGGCGTGCCTCCCAACGCGGTGACGGAGATCATGAACGGCGAGCGCCGGATCA
CCGCCCCGATGGCGCTGAGGCTGGGCAAGGCCTTCGGAACCGACGCCCGCTATTGGACCAACCTGCAGAGCATCTTCGAG
ACCAAGGTCGCGCGGGCGGAGATCGGCGATGCGCTGGACGCCATCCAGCCGCTGCCGCATCCGGGCAAGGCGGCTTAA