Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4581709..4582311 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N1705_RS22020 Protein ID WP_000897305.1
Coordinates 4582000..4582311 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N1705_RS22015 Protein ID WP_000356397.1
Coordinates 4581709..4581999 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS21990 (4577654) 4577654..4578556 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N1705_RS21995 (4578553) 4578553..4579188 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N1705_RS22000 (4579185) 4579185..4580114 + 930 WP_032359289.1 formate dehydrogenase accessory protein FdhE -
N1705_RS22005 (4580444) 4580444..4580686 - 243 WP_001086388.1 protein YiiF -
N1705_RS22010 (4580905) 4580905..4581123 - 219 WP_001295676.1 CopG family transcriptional regulator -
N1705_RS22015 (4581709) 4581709..4581999 - 291 WP_000356397.1 NadS family protein Antitoxin
N1705_RS22020 (4582000) 4582000..4582311 - 312 WP_000897305.1 hypothetical protein Toxin
N1705_RS22025 (4582540) 4582540..4583448 + 909 WP_044722469.1 alpha/beta hydrolase -
N1705_RS22030 (4583616) 4583616..4584530 - 915 WP_260248703.1 transposase -
N1705_RS22035 (4584543) 4584543..4585430 - 888 Protein_4304 hypothetical protein -
N1705_RS22040 (4585845) 4585845..4586786 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N1705_RS22045 (4586831) 4586831..4587268 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T257284 WP_000897305.1 NZ_CP104123:c4582311-4582000 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT257284 WP_000356397.1 NZ_CP104123:c4581999-4581709 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References