257265

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 709578..710305 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N1705_RS03460 Protein ID WP_000550189.1
Coordinates 709578..709892 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N1705_RS03465 Protein ID WP_000560266.1
Coordinates 709889..710305 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS03440 (705744) 705744..706730 - 987 WP_032206007.1 Gfo/Idh/MocA family oxidoreductase -
N1705_RS03445 (706809) 706809..707492 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
N1705_RS03450 (707569) 707569..708072 - 504 WP_001297151.1 M48 family metallopeptidase -
N1705_RS03455 (708157) 708157..709293 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N1705_RS03460 (709578) 709578..709892 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N1705_RS03465 (709889) 709889..710305 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N1705_RS03470 (710350) 710350..712368 - 2019 WP_219411614.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N1705_RS03475 (712794) 712794..715145 - 2352 WP_219411615.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T257265 WP_000550189.1 NZ_CP104123:709578-709892 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT257265 WP_000560266.1 NZ_CP104123:709889-710305 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References