257258

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4763124..4763726 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N1709_RS23195 Protein ID WP_000897305.1
Coordinates 4763415..4763726 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N1709_RS23190 Protein ID WP_000356397.1
Coordinates 4763124..4763414 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS23165 (4759049) 4759049..4759951 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N1709_RS23170 (4759948) 4759948..4760583 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N1709_RS23175 (4760580) 4760580..4761509 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
N1709_RS23180 (4761839) 4761839..4762081 - 243 WP_001087409.1 protein YiiF -
N1709_RS23185 (4762301) 4762301..4762519 - 219 WP_001315930.1 CopG family transcriptional regulator -
N1709_RS23190 (4763124) 4763124..4763414 - 291 WP_000356397.1 NadS family protein Antitoxin
N1709_RS23195 (4763415) 4763415..4763726 - 312 WP_000897305.1 hypothetical protein Toxin
N1709_RS23200 (4763955) 4763955..4764863 + 909 WP_001162704.1 alpha/beta hydrolase -
N1709_RS23205 (4764927) 4764927..4765868 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N1709_RS23210 (4765913) 4765913..4766350 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N1709_RS23215 (4766347) 4766347..4767219 - 873 WP_000920762.1 virulence factor BrkB family protein -
N1709_RS23220 (4767213) 4767213..4767812 - 600 WP_001295269.1 glucose-1-phosphatase -
N1709_RS23225 (4767911) 4767911..4768696 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T257258 WP_000897305.1 NZ_CP104117:c4763726-4763415 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT257258 WP_000356397.1 NZ_CP104117:c4763414-4763124 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References