Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3911669..3912210 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag N1709_RS19240 Protein ID WP_000615976.1
Coordinates 3911932..3912210 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N1709_RS19235 Protein ID WP_000729704.1
Coordinates 3911669..3911929 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS19210 (3907452) 3907452..3908237 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
N1709_RS19215 (3908209) 3908209..3909921 + 1713 Protein_3767 flagellar biosynthesis protein FlhA -
N1709_RS19220 (3910026) 3910026..3910304 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
N1709_RS19225 (3910297) 3910297..3910653 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
N1709_RS19230 (3910710) 3910710..3911483 - 774 WP_000543895.1 C40 family peptidase -
N1709_RS19235 (3911669) 3911669..3911929 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N1709_RS19240 (3911932) 3911932..3912210 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N1709_RS19245 (3912366) 3912366..3913106 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N1709_RS19250 (3913077) 3913077..3913844 - 768 WP_000333380.1 class II glutamine amidotransferase -
N1709_RS19255 (3914050) 3914050..3914628 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3910297..3928195 17898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T257254 WP_000615976.1 NZ_CP104117:3911932-3912210 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT257254 WP_000729704.1 NZ_CP104117:3911669-3911929 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References