257239

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 674152..674951 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag N1709_RS03295 Protein ID WP_000347266.1
Coordinates 674152..674616 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N1709_RS03300 Protein ID WP_001307405.1
Coordinates 674616..674951 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS03265 (669153) 669153..669587 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N1709_RS03270 (669605) 669605..670483 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
N1709_RS03275 (670473) 670473..671252 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N1709_RS03280 (671263) 671263..671736 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N1709_RS03285 (671759) 671759..673039 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
N1709_RS03290 (673288) 673288..674097 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N1709_RS03295 (674152) 674152..674616 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N1709_RS03300 (674616) 674616..674951 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N1709_RS03305 (675100) 675100..676671 - 1572 WP_001273741.1 galactarate dehydratase -
N1709_RS03310 (677046) 677046..678380 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N1709_RS03315 (678396) 678396..679166 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T257239 WP_000347266.1 NZ_CP104117:c674616-674152 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257239 WP_001307405.1 NZ_CP104117:c674951-674616 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References