Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 920234..920928 Replicon chromosome
Accession NZ_CP104112
Organism Escherichia coli strain USDA-ARS-USMARC-51688

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag N1707_RS04400 Protein ID WP_001263493.1
Coordinates 920530..920928 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N1707_RS04395 Protein ID WP_000554757.1
Coordinates 920234..920527 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1707_RS04380 (917267) 917267..918130 + 864 WP_001169518.1 flagellar motor stator protein MotA -
N1707_RS04385 (918133) 918133..919056 + 924 WP_112032734.1 putative lateral flagellar export/assembly protein LafU -
N1707_RS04390 (919127) 919127..920182 + 1056 WP_001226164.1 DNA polymerase IV -
N1707_RS04395 (920234) 920234..920527 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N1707_RS04400 (920530) 920530..920928 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N1707_RS04405 (920938) 920938..921390 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
N1707_RS04410 (921636) 921636..921839 + 204 Protein_839 RtcB family protein -
N1707_RS04415 (921838) 921838..922359 + 522 Protein_840 peptide chain release factor H -
N1707_RS04420 (922416) 922416..923873 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
N1707_RS04425 (924134) 924134..924592 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (925188) 925188..925268 + 81 NuclAT_10 - -
- (925188) 925188..925268 + 81 NuclAT_10 - -
- (925188) 925188..925268 + 81 NuclAT_10 - -
- (925188) 925188..925268 + 81 NuclAT_10 - -
N1707_RS04430 (924684) 924684..925928 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 916478..916981 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T257195 WP_001263493.1 NZ_CP104112:920530-920928 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT257195 WP_000554757.1 NZ_CP104112:920234-920527 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References