Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3409414..3410093 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag N0M81_RS16315 Protein ID WP_000057541.1
Coordinates 3409791..3410093 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N0M81_RS16310 Protein ID WP_000806442.1
Coordinates 3409414..3409755 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS16300 (3405657) 3405657..3406589 - 933 WP_000883039.1 glutaminase A -
N0M81_RS16305 (3406852) 3406852..3409356 + 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
N0M81_RS16310 (3409414) 3409414..3409755 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N0M81_RS16315 (3409791) 3409791..3410093 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N0M81_RS16320 (3410226) 3410226..3411020 + 795 WP_000365157.1 TraB/GumN family protein -
N0M81_RS16325 (3411224) 3411224..3411703 + 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N0M81_RS16330 (3411740) 3411740..3413392 - 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
N0M81_RS16335 (3413610) 3413610..3414830 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T257127 WP_000057541.1 NZ_CP104077:c3410093-3409791 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT257127 WP_000806442.1 NZ_CP104077:c3409755-3409414 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References