Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2262279..2262841 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag N0M81_RS10705 Protein ID WP_000605676.1
Coordinates 2262563..2262841 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag N0M81_RS10700 Protein ID WP_000781370.1
Coordinates 2262279..2262563 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS10685 (2257638) 2257638..2260685 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
N0M81_RS10690 (2260698) 2260698..2261582 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
N0M81_RS10695 (2261575) 2261575..2262228 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
N0M81_RS10700 (2262279) 2262279..2262563 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
N0M81_RS10705 (2262563) 2262563..2262841 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N0M81_RS10710 (2263027) 2263027..2264037 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
N0M81_RS10715 (2264171) 2264171..2265868 - 1698 WP_000433464.1 malate dehydrogenase -
N0M81_RS10720 (2266025) 2266025..2266162 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
N0M81_RS10725 (2266264) 2266264..2266479 - 216 WP_000495766.1 biofilm-dependent modulation protein -
N0M81_RS10730 (2266824) 2266824..2267255 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T257121 WP_000605676.1 NZ_CP104077:c2262841-2262563 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT257121 WP_000781370.1 NZ_CP104077:c2262563-2262279 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References