Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 678805..679604 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag N0M81_RS03305 Protein ID WP_000347273.1
Coordinates 678805..679269 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag N0M81_RS03310 Protein ID WP_001308975.1
Coordinates 679269..679604 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS03275 (673806) 673806..674240 - 435 WP_000948834.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N0M81_RS03280 (674258) 674258..675136 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
N0M81_RS03285 (675126) 675126..675905 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N0M81_RS03290 (675916) 675916..676389 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N0M81_RS03295 (676412) 676412..677692 - 1281 WP_000681961.1 tagatose-bisphosphate aldolase subunit KbaZ -
N0M81_RS03300 (677941) 677941..678750 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N0M81_RS03305 (678805) 678805..679269 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N0M81_RS03310 (679269) 679269..679604 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N0M81_RS03315 (679753) 679753..681324 - 1572 WP_001273756.1 galactarate dehydratase -
N0M81_RS03320 (681699) 681699..683033 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
N0M81_RS03325 (683049) 683049..683819 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 678805..690085 11280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T257116 WP_000347273.1 NZ_CP104077:c679269-678805 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT257116 WP_001308975.1 NZ_CP104077:c679604-679269 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References