Detailed information of TA system TA025698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 214177..214723
• Replicon: chromosome
• Accession: NC_016147
• Organism: Pseudoxanthomonas spadix BD-a59

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DSC_RS00910
• Protein ID: WP_202944898.1
• Coordinates: 214484..214723 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DSC_RS15905
• Protein ID: WP_014159221.1
• Coordinates: 214177..214491 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSC_RS00890	210049..210792	+	744	WP_014159218.1	ATP-binding cassette domain-containing protein	-
DSC_RS00895	210789..212273	+	1485	WP_014159219.1	ABC transporter permease subunit	-
DSC_RS16970	212276..212560	+	285	WP_014159220.1	hypothetical protein	-
DSC_RS16440	213194..213817	-	624	WP_158308221.1	hypothetical protein	-
DSC_RS16445	213860..214048	+	189	Protein_187	IS110 family transposase	-
DSC_RS15905	214177..214491	-	315	WP_014159221.1	helix-turn-helix domain-containing protein	Antitoxin
DSC_RS00910	214484..214723	-	240	WP_202944898.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSC_RS00915	214913..215500	-	588	WP_014159224.1	hypothetical protein	-
DSC_RS17035	215759..215896	+	138	WP_014159225.1	hypothetical protein	-
DSC_RS00920	215893..217068	-	1176	WP_014159226.1	aminotransferase class V-fold PLP-dependent enzyme	-
DSC_RS00925	217140..218435	+	1296	WP_014159227.1	ergothioneine biosynthesis protein EgtB	-
DSC_RS00930	218432..219418	+	987	WP_014159228.1	L-histidine N(alpha)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	206710..215896	9186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (3-74)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-96)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 8943.60 Da
• Isoelectric Point: 11.1190

>T25698 WP_202944898.1 NC_016147:c214723-214484 [Pseudoxanthomonas spadix BD-a59]
MAELLAEHGPSLRLPHSRALGDGLFELRPRGRSGIGRALYCFLLGKRIVMVHAFIKKTQETPDKELKLARKRVKELQHG

Nucleotide

• Length: 240 bp

>T25698 NC_016147:c214723-214484 [Pseudoxanthomonas spadix BD-a59]
TTGGCTGAGCTACTGGCTGAGCATGGCCCCAGCCTTCGCCTGCCTCACTCCAGGGCCCTCGGGGATGGGCTATTCGAGCT
GAGGCCACGTGGGCGCTCCGGCATTGGTCGTGCCCTCTACTGCTTCTTGCTTGGCAAGCGCATCGTGATGGTCCACGCCT
TCATCAAGAAAACACAAGAAACACCCGACAAAGAACTCAAGCTGGCCCGTAAACGGGTCAAGGAGCTGCAGCATGGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11576.34 Da
• Isoelectric Point: 10.3556

>AT25698 WP_014159221.1 NC_016147:c214491-214177 [Pseudoxanthomonas spadix BD-a59]
MANLKYRPVVHDHQEFLARASKRKGFAEAYEALELEYELANQMLKARSRAGLTQDAVAERMGTTKSAISRLESAGKKNAP
SIATLQRYARAVGCDLQVKLIPQK

Nucleotide

• Length: 315 bp

>AT25698 NC_016147:c214491-214177 [Pseudoxanthomonas spadix BD-a59]
ATGGCTAACCTCAAATACAGGCCCGTGGTACATGACCACCAGGAATTCCTGGCTCGCGCATCCAAGCGCAAGGGATTTGC
TGAGGCTTATGAAGCTTTGGAGTTGGAGTATGAGCTTGCCAACCAAATGCTCAAGGCCCGCTCGCGAGCGGGTCTTACCC
AAGATGCCGTCGCTGAGCGCATGGGCACTACAAAAAGCGCCATTTCGCGGCTTGAATCTGCTGGCAAAAAAAATGCGCCA
TCCATTGCTACTCTCCAAAGATATGCGCGGGCGGTTGGCTGCGATCTACAAGTCAAACTGATTCCACAGAAGTGA