Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4523577..4524208 Replicon chromosome
Accession NC_016112
Organism Methylomicrobium alcaliphilum 20Z

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MEALZ_RS19265 Protein ID WP_198482321.1
Coordinates 4523882..4524208 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MEALZ_RS19260 Protein ID WP_014150329.1
Coordinates 4523577..4523867 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MEALZ_RS19240 4520432..4521112 + 681 WP_014150325.1 SDR family oxidoreductase -
MEALZ_RS19245 4521184..4522446 + 1263 WP_046061712.1 FAD-binding oxidoreductase -
MEALZ_RS19250 4522726..4522926 - 201 WP_014150327.1 hypothetical protein -
MEALZ_RS19255 4522916..4523185 - 270 Protein_3759 BrnT family toxin -
MEALZ_RS19260 4523577..4523867 - 291 WP_014150329.1 HigA family addiction module antidote protein Antitoxin
MEALZ_RS19265 4523882..4524208 - 327 WP_198482321.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MEALZ_RS23210 4524541..4524891 - 351 WP_198482323.1 hypothetical protein -
MEALZ_RS19280 4525693..4526244 - 552 WP_014150334.1 hypothetical protein -
MEALZ_RS19285 4526542..4527243 - 702 WP_014150335.1 urate hydroxylase PuuD -
MEALZ_RS19290 4527886..4528749 - 864 WP_014150337.1 RnfABCDGE type electron transport complex subunit B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(16-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12452.18 Da        Isoelectric Point: 7.5222

>T25696 WP_198482321.1 NC_016112:c4524208-4523882 [Methylotuvimicrobium alcaliphilum 20Z]
MHEYRESVNIGIMIVSFSDKATSDLFHGISSNKARKLPNQIHELALYKLDIINAAQNLDDLKSPPANRLEPLKGDLKGFH
SIRINNQWRIVFRWEANAAHDVQIMDYH

Download         Length: 327 bp

>T25696 NC_016112:c4524208-4523882 [Methylotuvimicrobium alcaliphilum 20Z]
TTGCATGAATACCGTGAATCCGTTAATATCGGCATCATGATTGTTTCATTCAGTGATAAGGCCACTTCAGACTTGTTTCA
CGGAATTTCCAGCAACAAAGCTAGAAAACTGCCCAACCAGATTCATGAATTGGCGCTGTATAAGCTGGACATAATCAATG
CTGCTCAAAACCTTGACGATCTTAAATCTCCGCCTGCAAACAGACTGGAACCACTCAAAGGTGATCTCAAGGGTTTTCAC
AGTATCCGTATAAACAATCAATGGCGCATTGTTTTCCGTTGGGAGGCCAATGCAGCTCATGATGTCCAAATTATGGACTA
TCACTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10709.27 Da        Isoelectric Point: 6.6420

>AT25696 WP_014150329.1 NC_016112:c4523867-4523577 [Methylotuvimicrobium alcaliphilum 20Z]
MIPTNRTATHPGTILLKEFLEPLEITQKALAIHLGISVQRVNEIVRGKRGITPDTAWLLSEAFNTSPEFWLNLQSTHDLS
AHRPEYHVESLVAAGI

Download         Length: 291 bp

>AT25696 NC_016112:c4523867-4523577 [Methylotuvimicrobium alcaliphilum 20Z]
ATGATTCCGACGAACAGAACTGCAACACACCCAGGTACGATATTACTTAAAGAGTTTCTTGAGCCTCTTGAGATTACCCA
AAAAGCCCTGGCTATTCATTTAGGTATATCCGTTCAACGCGTTAATGAGATTGTACGTGGTAAACGTGGTATTACGCCAG
ATACTGCCTGGCTACTTTCTGAGGCTTTCAATACGTCCCCTGAATTTTGGCTTAATCTGCAATCGACTCATGATCTGTCG
GCTCATAGACCTGAGTATCATGTCGAGTCTTTGGTTGCTGCGGGCATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References