Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3888992..3889686 Replicon chromosome
Accession NZ_CP103974
Organism Escherichia coli strain DLI.6n

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag NZ657_RS19030 Protein ID WP_001263488.1
Coordinates 3888992..3889390 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NZ657_RS19035 Protein ID WP_000554758.1
Coordinates 3889393..3889686 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3884580) 3884580..3884660 - 81 NuclAT_12 - -
- (3884580) 3884580..3884660 - 81 NuclAT_12 - -
- (3884580) 3884580..3884660 - 81 NuclAT_12 - -
- (3884580) 3884580..3884660 - 81 NuclAT_12 - -
NZ657_RS19005 (3885256) 3885256..3885714 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NZ657_RS19010 (3885975) 3885975..3887432 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NZ657_RS19015 (3887489) 3887489..3888010 - 522 Protein_3720 peptide chain release factor H -
NZ657_RS19020 (3888006) 3888006..3888212 - 207 Protein_3721 RtcB family protein -
NZ657_RS19025 (3888530) 3888530..3888982 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NZ657_RS19030 (3888992) 3888992..3889390 - 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NZ657_RS19035 (3889393) 3889393..3889686 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NZ657_RS19040 (3889738) 3889738..3890793 - 1056 WP_001226164.1 DNA polymerase IV -
NZ657_RS19045 (3890864) 3890864..3891649 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NZ657_RS19050 (3891621) 3891621..3893333 + 1713 Protein_3727 flagellar biosynthesis protein FlhA -
NZ657_RS19055 (3893557) 3893557..3894054 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3857909..3894856 36947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T256950 WP_001263488.1 NZ_CP103974:c3889390-3888992 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT256950 WP_000554758.1 NZ_CP103974:c3889686-3889393 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References