Detailed information of TA system TA025694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 4378386..4378918
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: G4SYZ3
• Locus tag: MEALZ_RS18595
• Protein ID: WP_014150203.1
• Coordinates: 4378386..4378655 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: G4SYZ4
• Locus tag: MEALZ_RS18600
• Protein ID: WP_014150204.1
• Coordinates: 4378658..4378918 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS18580	4374479..4375648	+	1170	WP_014150199.1	acyl-CoA dehydrogenase family protein	-
MEALZ_RS18585	4375710..4376522	+	813	Protein_3633	class I SAM-dependent methyltransferase	-
MEALZ_RS18590	4376983..4377996	+	1014	WP_046061698.1	fructose-bisphosphate aldolase class II	-
MEALZ_RS18595	4378386..4378655	-	270	WP_014150203.1	Txe/YoeB family addiction module toxin	Toxin
MEALZ_RS18600	4378658..4378918	-	261	WP_014150204.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MEALZ_RS18605	4379132..4379380	+	249	WP_014150205.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MEALZ_RS18610	4379370..4379669	+	300	WP_014150206.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MEALZ_RS22860	4379700..4379870	+	171	Protein_3639	addiction module toxin RelE	-
MEALZ_RS18615	4380016..4380264	+	249	WP_198482315.1	type II toxin-antitoxin system ParD family antitoxin	-
MEALZ_RS18620	4380264..4380560	+	297	WP_014150208.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MEALZ_RS18625	4380615..4381466	+	852	WP_014150209.1	transposase	-
MEALZ_RS21090	4381548..4383458	-	1911	WP_014150210.1	acyltransferase	-
MEALZ_RS18635	4383577..4383916	-	340	Protein_3644	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10627.07 Da
• Isoelectric Point: 8.8987

>T25694 WP_014150203.1 NC_016112:c4378655-4378386 [Methylotuvimicrobium alcaliphilum 20Z]
MRAIRFVPDAWEDYLYWQHQDNKTLKRLNLLITASARDPFTGIGKLEPLRGDLSGYWSRRIDDTNRLVYRATDTELVIIA
CRFHYESRH

Nucleotide

• Length: 270 bp

>T25694 NC_016112:c4378655-4378386 [Methylotuvimicrobium alcaliphilum 20Z]
ATGCGTGCCATTCGATTTGTTCCCGATGCATGGGAGGATTATCTGTACTGGCAACATCAGGATAACAAAACGCTCAAGCG
GTTAAACCTCTTGATTACAGCCTCTGCGCGCGATCCGTTTACCGGAATCGGAAAGCTCGAACCGCTACGTGGCGATTTAT
CCGGTTACTGGTCACGACGCATAGATGATACAAACCGCCTGGTATATCGGGCAACCGATACAGAGTTAGTCATTATCGCT
TGCCGTTTTCATTACGAGAGCCGTCATTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9298.45 Da
• Isoelectric Point: 5.4925

>AT25694 WP_014150204.1 NC_016112:c4378918-4378658 [Methylotuvimicrobium alcaliphilum 20Z]
MKVVTYSHARNALKSILDEVVKDADVIIISRRDAEGDAVVMSLDSYNSIMETLHLTSNPANAAALARAIAQDKAGQAQEH
QLHPSD

Nucleotide

• Length: 261 bp

>AT25694 NC_016112:c4378918-4378658 [Methylotuvimicrobium alcaliphilum 20Z]
ATGAAAGTCGTTACCTACTCCCATGCACGCAATGCTCTCAAGTCTATATTGGATGAGGTAGTAAAAGATGCTGACGTTAT
CATTATCAGTCGTCGTGATGCAGAAGGTGATGCAGTGGTGATGTCGCTGGATAGCTACAACAGCATCATGGAGACATTGC
ACCTGACTAGTAATCCAGCGAACGCGGCGGCATTAGCCAGAGCCATCGCACAAGACAAAGCGGGACAGGCGCAAGAACAT
CAATTACACCCTAGTGATTAA

Structures

Toxin

• AlphaFold DB: G4SYZ3

Antitoxin

• AlphaFold DB: G4SYZ4