Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 633551..634350 Replicon chromosome
Accession NZ_CP103974
Organism Escherichia coli strain DLI.6n

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag NZ657_RS03070 Protein ID WP_000347275.1
Coordinates 633551..634015 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NZ657_RS03075 Protein ID WP_001307405.1
Coordinates 634015..634350 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZ657_RS03040 (628552) 628552..628986 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
NZ657_RS03045 (629004) 629004..629882 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
NZ657_RS03050 (629872) 629872..630651 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
NZ657_RS03055 (630662) 630662..631135 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
NZ657_RS03060 (631158) 631158..632438 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NZ657_RS03065 (632687) 632687..633496 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NZ657_RS03070 (633551) 633551..634015 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NZ657_RS03075 (634015) 634015..634350 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NZ657_RS03080 (634499) 634499..636070 - 1572 WP_001273753.1 galactarate dehydratase -
NZ657_RS03085 (636445) 636445..637779 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NZ657_RS03090 (637795) 637795..638565 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T256936 WP_000347275.1 NZ_CP103974:c634015-633551 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT256936 WP_001307405.1 NZ_CP103974:c634350-634015 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References