Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3836306..3837000 Replicon chromosome
Accession NZ_CP103972
Organism Escherichia coli strain DLI.5a

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NZD79_RS18680 Protein ID WP_001263489.1
Coordinates 3836602..3837000 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NZD79_RS18675 Protein ID WP_000554758.1
Coordinates 3836306..3836599 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NZD79_RS18655 (3831938) 3831938..3832435 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NZD79_RS18660 (3832659) 3832659..3834371 - 1713 Protein_3638 flagellar biosynthesis protein FlhA -
NZD79_RS18665 (3834343) 3834343..3835128 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NZD79_RS18670 (3835199) 3835199..3836254 + 1056 WP_001226164.1 DNA polymerase IV -
NZD79_RS18675 (3836306) 3836306..3836599 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NZD79_RS18680 (3836602) 3836602..3837000 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NZD79_RS18685 (3837079) 3837079..3837776 + 698 WP_248783592.1 IS1 family transposase -
NZD79_RS18690 (3837778) 3837778..3838239 + 462 WP_259568154.1 GNAT family N-acetyltransferase -
NZD79_RS18695 (3838557) 3838557..3838763 + 207 Protein_3645 RtcB family protein -
NZD79_RS18700 (3838759) 3838759..3839280 + 522 Protein_3646 peptide chain release factor H -
NZD79_RS18705 (3839337) 3839337..3840794 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NZD79_RS18710 (3841055) 3841055..3841513 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3831136..3869504 38368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T256933 WP_001263489.1 NZ_CP103972:3836602-3837000 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT256933 WP_000554758.1 NZ_CP103972:3836306-3836599 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References