Detailed information of TA system TA256880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3962867..3963582
• Replicon: chromosome
• Accession: NZ_CP103872
• Organism: Herbaspirillum huttiense strain ZXN111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: NY669_RS17710
• Protein ID: WP_259435300.1
• Coordinates: 3963184..3963582 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NY669_RS17705
• Protein ID: WP_034329811.1
• Coordinates: 3962867..3963187 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NY669_RS17685 (NY669_17685)	3959044..3959706	-	663	WP_259432797.1	response regulator transcription factor	-
NY669_RS17690 (NY669_17690)	3959703..3960905	-	1203	WP_259432798.1	PAS domain-containing sensor histidine kinase	-
NY669_RS17695 (NY669_17695)	3960961..3961722	-	762	WP_209534462.1	glucose 1-dehydrogenase	-
NY669_RS17700 (NY669_17700)	3961944..3962663	-	720	WP_034329812.1	FadR/GntR family transcriptional regulator	-
NY669_RS17705 (NY669_17705)	3962867..3963187	-	321	WP_034329811.1	transcriptional regulator	Antitoxin
NY669_RS17710 (NY669_17710)	3963184..3963582	-	399	WP_259435300.1	toxin	Toxin
NY669_RS17715 (NY669_17715)	3963582..3964910	-	1329	WP_121045052.1	MFS transporter	-
NY669_RS17720 (NY669_17720)	3965000..3966202	-	1203	WP_134222985.1	M20 aminoacylase family protein	-
NY669_RS17725 (NY669_17725)	3966539..3967519	+	981	WP_259432799.1	LysR family transcriptional regulator	-
NY669_RS17730 (NY669_17730)	3967576..3968481	+	906	WP_259432800.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3950552..3978857	28305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-89)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15635.92 Da
• Isoelectric Point: 9.5617

>T256880 WP_259435300.1 NZ_CP103872:c3963582-3963184 [Herbaspirillum huttiense]
MLHYGTMANYTRDAYGSRMEAIFKETRMFAAVRENYLSYDDFATLQQMLMRQPQAGSLIKDAGGLRKIRFPDARRCKGKR
GGLRVIYYFWDEQCHFWLCSIYDKDEMNDLTAHQRKLLRRALEDELSSRKTS

Nucleotide

• Length: 399 bp

>T256880 NZ_CP103872:c3963582-3963184 [Herbaspirillum huttiense]
ATGCTGCACTATGGTACGATGGCGAACTATACGCGAGATGCGTATGGTTCGCGGATGGAAGCGATCTTCAAAGAAACGCG
AATGTTTGCAGCAGTCCGGGAGAACTACCTCTCGTACGATGACTTTGCAACGCTTCAGCAGATGCTGATGCGACAGCCGC
AAGCGGGAAGCCTCATCAAGGATGCGGGGGGACTTCGAAAGATTCGTTTTCCTGATGCGCGTCGTTGCAAGGGAAAACGT
GGTGGCCTGAGAGTGATCTACTATTTTTGGGACGAACAATGCCACTTCTGGCTTTGCTCTATCTACGACAAAGATGAGAT
GAACGACCTCACCGCTCATCAGAGAAAACTGCTCAGGCGTGCGCTGGAAGATGAACTGTCCTCAAGGAAGACATCATGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11961.89 Da
• Isoelectric Point: 10.8930

>AT256880 WP_034329811.1 NZ_CP103872:c3963187-3962867 [Herbaspirillum huttiense]
MKKRNLFEELMAGVQDMADEREGKLTLRTSKVKLSPEPVTITAEEIQRVRGKARVSQAIMARSLRVNPRTYQNWEQGVSR
PNAQAAVLLKLVDQDPTLFARISALV

Nucleotide

• Length: 321 bp

>AT256880 NZ_CP103872:c3963187-3962867 [Herbaspirillum huttiense]
ATGAAAAAAAGAAACTTATTCGAAGAGCTGATGGCGGGCGTGCAAGACATGGCCGATGAGCGAGAGGGCAAGCTGACCTT
GCGCACAAGCAAAGTCAAGCTATCGCCGGAGCCGGTCACGATCACAGCCGAGGAGATTCAACGGGTACGTGGCAAGGCTC
GGGTGTCGCAGGCGATCATGGCCCGCAGCCTGCGTGTCAATCCGCGGACGTATCAAAACTGGGAGCAGGGCGTATCACGG
CCCAACGCGCAGGCAGCCGTGTTGCTCAAGCTGGTGGACCAAGACCCCACGCTCTTCGCACGGATCAGCGCATTGGTGTG
A