Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3062022..3062716 Replicon chromosome
Accession NZ_CP103864
Organism Escherichia coli O157:H7 strain RV06

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag NUE47_RS14695 Protein ID WP_001263495.1
Coordinates 3062318..3062716 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag NUE47_RS14690 Protein ID WP_000554757.1
Coordinates 3062022..3062315 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE47_RS14670 (3057733) 3057733..3058230 + 498 Protein_2846 REP-associated tyrosine transposase RayT -
NUE47_RS14675 (3058375) 3058375..3060087 - 1713 Protein_2847 flagellar biosynthesis protein FlhA -
NUE47_RS14680 (3060059) 3060059..3060844 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
NUE47_RS14685 (3060915) 3060915..3061970 + 1056 WP_001226188.1 DNA polymerase IV -
NUE47_RS14690 (3062022) 3062022..3062315 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NUE47_RS14695 (3062318) 3062318..3062716 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NUE47_RS14700 (3062726) 3062726..3063178 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
NUE47_RS14705 (3063497) 3063497..3063700 + 204 Protein_2853 RtcB family protein -
NUE47_RS14710 (3063699) 3063699..3064220 + 522 Protein_2854 peptide chain release factor H -
NUE47_RS14715 (3064277) 3064277..3065734 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
NUE47_RS14720 (3065995) 3065995..3066453 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (3067049) 3067049..3067129 + 81 NuclAT_12 - -
- (3067049) 3067049..3067129 + 81 NuclAT_12 - -
- (3067049) 3067049..3067129 + 81 NuclAT_12 - -
- (3067049) 3067049..3067129 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T256858 WP_001263495.1 NZ_CP103864:3062318-3062716 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT256858 WP_000554757.1 NZ_CP103864:3062022-3062315 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References