Detailed information of TA system TA025684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2022934..2023525
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G4T106
• Locus tag: MEALZ_RS08570
• Protein ID: WP_014148233.1
• Coordinates: 2022934..2023245 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MEALZ_RS08575
• Protein ID: WP_014148234.1
• Coordinates: 2023238..2023525 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS08530	2017992..2018384	+	393	WP_014148228.1	formamidase	-
MEALZ_RS08540	2019174..2019380	+	207	WP_014148229.1	hypothetical protein	-
MEALZ_RS08545	2019373..2019594	+	222	WP_046061064.1	type II toxin-antitoxin system HicA family toxin	-
MEALZ_RS08550	2019665..2019907	+	243	WP_046061065.1	hypothetical protein	-
MEALZ_RS08555	2019909..2021150	-	1242	WP_014148230.1	hypothetical protein	-
MEALZ_RS08560	2021234..2021434	-	201	WP_014148231.1	hypothetical protein	-
MEALZ_RS08565	2021724..2022173	+	450	WP_014148232.1	hypothetical protein	-
MEALZ_RS21780	2022469..2022708	+	240	WP_084685383.1	hypothetical protein	-
MEALZ_RS08570	2022934..2023245	+	312	WP_014148233.1	transcriptional regulator	Toxin
MEALZ_RS08575	2023238..2023525	+	288	WP_014148234.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
MEALZ_RS08580	2023892..2024146	+	255	WP_014148235.1	hypothetical protein	-
MEALZ_RS08585	2024146..2024463	+	318	WP_046061066.1	type II toxin-antitoxin system PemK/MazF family toxin	-
MEALZ_RS08590	2024732..2025229	-	498	WP_046061067.1	GNAT family N-acetyltransferase	-
MEALZ_RS08595	2025226..2025498	-	273	WP_014148237.1	DUF1778 domain-containing protein	-
MEALZ_RS08605	2026100..2027335	+	1236	WP_014148238.1	hypothetical protein	-
MEALZ_RS08610	2027360..2028028	+	669	Protein_1679	transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2028082..2028900	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11768.46 Da
• Isoelectric Point: 8.3885

>T25684 WP_014148233.1 NC_016112:2022934-2023245 [Methylotuvimicrobium alcaliphilum 20Z]
MIFIETSTFTKMLPNYLSDEEYQGLQTYLMQKPDAGDIVKGSGGVRKVRWASEGKGKSSGVRAIYYWKKPDHEIWMLTIY
SKSERSTIPGHILKQIAEAIDHD

Nucleotide

• Length: 312 bp

>T25684 NC_016112:2022934-2023245 [Methylotuvimicrobium alcaliphilum 20Z]
ATGATATTTATCGAAACCAGTACATTTACAAAGATGCTTCCGAATTACCTCTCAGATGAAGAGTATCAGGGGTTGCAGAC
ATATTTGATGCAAAAACCGGATGCTGGAGATATCGTTAAAGGATCTGGAGGTGTAAGAAAGGTTCGGTGGGCTTCTGAAG
GTAAAGGAAAGAGTTCAGGTGTTCGTGCAATATACTATTGGAAAAAGCCTGATCATGAAATTTGGATGCTTACGATTTAC
AGTAAATCGGAGCGATCAACAATTCCAGGGCATATTTTGAAACAAATCGCTGAGGCAATAGATCATGACTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10662.34 Da
• Isoelectric Point: 10.0886

>AT25684 WP_014148234.1 NC_016112:2023238-2023525 [Methylotuvimicrobium alcaliphilum 20Z]
MTKRNIGLEILDGIREIKEHNNGNLQLKVTELSEPSPPKIIRAKLKMSQSAFAGLLGVSMRTLQDWEQGRREPQGPAVAL
LRIAEQHPEVFGQLH

Nucleotide

• Length: 288 bp

>AT25684 NC_016112:2023238-2023525 [Methylotuvimicrobium alcaliphilum 20Z]
ATGACTAAACGAAATATCGGTCTTGAAATCTTGGATGGTATTAGAGAGATAAAAGAGCATAATAATGGTAACTTGCAGTT
AAAGGTGACAGAGCTTTCTGAGCCTTCTCCTCCTAAAATTATTCGGGCAAAACTAAAAATGTCACAATCTGCATTTGCTG
GATTGTTAGGTGTAAGCATGCGCACTCTTCAAGATTGGGAACAAGGCCGCAGAGAGCCACAAGGTCCTGCTGTTGCACTG
CTTCGTATTGCGGAACAGCATCCCGAAGTTTTTGGGCAACTTCACTAA

Structures

Toxin

• AlphaFold DB: G4T106