Detailed information of TA system TA025681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 508925..509518
• Replicon: chromosome
• Accession: NC_016112
• Organism: Methylomicrobium alcaliphilum 20Z

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G4SYG6
• Locus tag: MEALZ_RS02300
• Protein ID: WP_014146976.1
• Coordinates: 509240..509518 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MEALZ_RS02295
• Protein ID: WP_014146975.1
• Coordinates: 508925..509227 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MEALZ_RS02270	504017..504955	+	939	WP_014146970.1	Nudix family hydrolase	-
MEALZ_RS02275	506297..507577	+	1281	WP_014146971.1	hypothetical protein	-
MEALZ_RS02280	507668..508090	+	423	WP_162472905.1	hypothetical protein	-
MEALZ_RS02285	508224..508496	+	273	WP_014146973.1	type II toxin-antitoxin system ParD family antitoxin	-
MEALZ_RS02290	508505..508831	+	327	WP_014146974.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MEALZ_RS02295	508925..509227	-	303	WP_014146975.1	HigA family addiction module antidote protein	Antitoxin
MEALZ_RS02300	509240..509518	-	279	WP_014146976.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MEALZ_RS02305	510313..511257	+	945	WP_014146977.1	hypothetical protein	-
MEALZ_RS02310	512232..512627	+	396	WP_014146978.1	hypothetical protein	-
MEALZ_RS02315	512802..513371	+	570	WP_014146979.1	DUF2939 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10579.07 Da
• Isoelectric Point: 10.1526

>T25681 WP_014146976.1 NC_016112:c509518-509240 [Methylotuvimicrobium alcaliphilum 20Z]
MIKSLRCKDTQALFEGESPKRFRAFQAIAERKLAQLHAAASLAFLRSPPGNRLEALKGSRAGQYSIRINDQWRVGFRWLD
NDAFEVEIVDYH

Nucleotide

• Length: 279 bp

>T25681 NC_016112:c509518-509240 [Methylotuvimicrobium alcaliphilum 20Z]
ATGATTAAGTCGCTTCGCTGCAAAGACACCCAAGCCTTGTTCGAAGGAGAATCGCCTAAACGTTTTCGCGCGTTTCAAGC
TATTGCCGAACGTAAGTTGGCGCAACTGCATGCCGCGGCTAGCCTGGCGTTTTTACGCAGCCCTCCGGGCAATCGCCTGG
AAGCGCTAAAAGGGAGTCGTGCGGGACAATACAGCATTCGCATCAATGACCAGTGGCGAGTGGGTTTTCGCTGGTTGGAT
AACGATGCTTTTGAGGTTGAAATTGTTGATTACCATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11356.99 Da
• Isoelectric Point: 7.3198

>AT25681 WP_014146975.1 NC_016112:c509227-508925 [Methylotuvimicrobium alcaliphilum 20Z]
MIENRMRPIHPGEILREDFLNPLALSVNGLATALHVPTTRLHEIVKERRAITPDTALRLARYFGGDAQSWLNLQTAYDLR
RAQQSSLDAIEREVLPREAA

Nucleotide

• Length: 303 bp

>AT25681 NC_016112:c509227-508925 [Methylotuvimicrobium alcaliphilum 20Z]
ATGATAGAAAACCGTATGCGTCCGATACACCCCGGCGAAATCCTGCGTGAAGATTTTTTGAATCCCTTGGCATTAAGCGT
AAACGGGTTGGCAACCGCTTTGCACGTTCCGACCACGCGTTTGCATGAAATCGTTAAGGAACGCCGCGCGATTACGCCGG
ATACCGCTTTGCGCTTGGCGCGTTATTTTGGCGGAGATGCACAATCCTGGCTGAACCTGCAAACAGCTTATGACTTACGC
CGGGCACAGCAATCCTCGCTTGACGCCATAGAGCGCGAGGTTTTGCCGCGCGAGGCGGCTTGA

Structures

Toxin

• AlphaFold DB: G4SYG6