Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 31594..32240 Replicon plasmid pMB9880_3
Accession NZ_CP103765
Organism Escherichia coli strain 5283

Toxin (Protein)


Gene name higB Uniprot ID A0A3P1YQM6
Locus tag M5T11_RS26275 Protein ID WP_025667955.1
Coordinates 31594..31941 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag M5T11_RS26280 Protein ID WP_001259436.1
Coordinates 31941..32240 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T11_RS26245 (M5T11_26245) 27086..27364 + 279 WP_032152803.1 hypothetical protein -
M5T11_RS26250 (M5T11_26250) 27374..28414 + 1041 WP_251296402.1 phage tail protein -
M5T11_RS26255 (M5T11_26255) 28416..28943 + 528 WP_032152674.1 tail fiber assembly protein -
M5T11_RS26260 (M5T11_26260) 29151..30131 + 981 WP_241336524.1 plasmid replication initiator RepA -
M5T11_RS26265 (M5T11_26265) 30775..31062 + 288 WP_032152672.1 hypothetical protein -
M5T11_RS26270 (M5T11_26270) 31086..31349 + 264 WP_000424604.1 hypothetical protein -
M5T11_RS26275 (M5T11_26275) 31594..31941 + 348 WP_025667955.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T11_RS26280 (M5T11_26280) 31941..32240 + 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
M5T11_RS26285 (M5T11_26285) 32405..32785 + 381 WP_000061763.1 hypothetical protein -
M5T11_RS26290 (M5T11_26290) 32849..33121 + 273 WP_000148349.1 helix-turn-helix domain-containing protein -
M5T11_RS26295 (M5T11_26295) 33732..33920 + 189 WP_004105254.1 hypothetical protein -
M5T11_RS26300 (M5T11_26300) 33931..35112 - 1182 WP_116290193.1 ORF6N domain-containing protein -
M5T11_RS26305 (M5T11_26305) 35109..35354 - 246 WP_250143293.1 hypothetical protein -
M5T11_RS26310 (M5T11_26310) 35331..35570 - 240 Protein_45 ash family protein -
M5T11_RS26315 (M5T11_26315) 36431..36679 - 249 WP_000730008.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48945 48945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13530.50 Da        Isoelectric Point: 9.3634

>T256612 WP_025667955.1 NZ_CP103765:31594-31941 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT256612 WP_001259436.1 NZ_CP103765:31941-32240 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P1YQM6

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References