Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3675084..3675778 Replicon chromosome
Accession NZ_CP103758
Organism Escherichia coli strain p10B

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NYO15_RS18075 Protein ID WP_001263489.1
Coordinates 3675084..3675482 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NYO15_RS18080 Protein ID WP_000554758.1
Coordinates 3675485..3675778 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3670672) 3670672..3670752 - 81 NuclAT_10 - -
- (3670672) 3670672..3670752 - 81 NuclAT_10 - -
- (3670672) 3670672..3670752 - 81 NuclAT_10 - -
- (3670672) 3670672..3670752 - 81 NuclAT_10 - -
NYO15_RS18050 (3671348) 3671348..3671806 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NYO15_RS18055 (3672067) 3672067..3673524 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NYO15_RS18060 (3673581) 3673581..3674102 - 522 Protein_3530 peptide chain release factor H -
NYO15_RS18065 (3674098) 3674098..3674304 - 207 Protein_3531 RtcB family protein -
NYO15_RS18070 (3674622) 3674622..3675074 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NYO15_RS18075 (3675084) 3675084..3675482 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NYO15_RS18080 (3675485) 3675485..3675778 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NYO15_RS18085 (3675830) 3675830..3676885 - 1056 WP_001226164.1 DNA polymerase IV -
NYO15_RS18090 (3676956) 3676956..3677741 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NYO15_RS18095 (3677713) 3677713..3679425 + 1713 Protein_3537 flagellar biosynthesis protein FlhA -
NYO15_RS18100 (3679649) 3679649..3680146 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T256571 WP_001263489.1 NZ_CP103758:c3675482-3675084 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT256571 WP_000554758.1 NZ_CP103758:c3675778-3675485 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References