Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3774219..3774898 Replicon chromosome
Accession NZ_CP103755
Organism Escherichia coli strain p11B

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag NYO14_RS18965 Protein ID WP_000057523.1
Coordinates 3774596..3774898 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag NYO14_RS18960 Protein ID WP_000806442.1
Coordinates 3774219..3774560 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NYO14_RS18950 (3770463) 3770463..3771395 - 933 WP_000883041.1 glutaminase A -
NYO14_RS18955 (3771657) 3771657..3774161 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
NYO14_RS18960 (3774219) 3774219..3774560 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
NYO14_RS18965 (3774596) 3774596..3774898 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NYO14_RS18970 (3775031) 3775031..3775825 + 795 WP_000365147.1 TraB/GumN family protein -
NYO14_RS18975 (3776029) 3776029..3776508 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
NYO14_RS18980 (3776676) 3776676..3777332 + 657 WP_015674862.1 hypothetical protein -
NYO14_RS18985 (3777329) 3777329..3777832 + 504 WP_000667000.1 hypothetical protein -
NYO14_RS18990 (3777870) 3777870..3779522 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T256538 WP_000057523.1 NZ_CP103755:c3774898-3774596 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT256538 WP_000806442.1 NZ_CP103755:c3774560-3774219 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References